Surface modification of chitosan film via polydopamine coating to promote biomineralization in bone tissue engineering

Chitosan-based material has been widely used as bone substitute due to its good biocompatibility and biodegradability. However, the hydrophobic surface of chitosan film constrains the osteogenesis mineralization in the process of bone regeneration. For this reason, we develop a novel polydopamine-modified chitosan film suitable for bone tissue engineering applications by a simple and feasible route in this study. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm the process of surface modification. For comparison, surface wettability, the capacity of mineralization in vitro, and biocompatibility of the chitosan film and the polydopamine-modified chitosan film were assessed. Research results indicate that the polydopamine-modified chitosan film has good hydrophilicity. It is very evident that the polydopamine treatment significantly influences the biomineralization capacity of the chitosan-based substrates, which enhance the growth rate of apatite on the modified chitosan film. Besides, MC3T3-E1 osteoblast experiments demonstrate that the cells can adhere and grow well on the polydopamine-modified chitosan film. It is anticipated that this polydopamine-modified chitosan film, which can be prepared in large quantities simply, should have potential applications in bone tissue engineering