Biotechnological potential of agro

This study aims at investigating pectin lyase bioproduction in submerged fermentation with synthetic medium and agro-industrial residues, using the filamentous fungus Aspergillus brasiliensis. The maximum pectin lyase activity in a synthetic medium (42？g/l pectin, 40？g/l yeast extract, and 0.02？g/l iron sulfate) was 31？U/ml, and 46？U/ml in the agro-industrial medium (160？g/l orange peel, 150？g/l corn steep liquor, and 300？g/l parboiled rice water), obtained over 60 and 124？h of bioproduction, 180？r/min, 30？℃, pHinitial 5.5, and 5·106 spores/ml, respectively. Partial characterization of pectin lyase crude enzyme extract obtained from the synthetic medium and the one made of agro-industrial residues showed optimum conditions at pH of 5.5 and 4.5 and temperatures of 37 and 55？℃, respectively. The Ed obtained was 3.13 and 9.15？kJ/mol, and the half-life time (t1/2) was 5.71 and 80？h at 55？℃ for pectin lyase produced in synthetic and agro-industrial medium, respectively