Influence of Different Methods Preparation on Platelet Activation in Stored Platelet Concentrates

Background: Platelet concentrates are routinely manufactured from whole blood by differential centrifugation. During this storage period under blood bank conditions, biochemical, structural and functional changes occur, a process that is also known as platelet storage lesion. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, CD63, lactate dehydrogenase and Annexin V. Materials and Methods: In this experimental study, 25 platelet concentrates prepared with platelet rich plasma-platelet concentrates, 25 units via buffy coat and apheresis-derived platelet methods. The percentages of Annexin V, CD63 expression, lactatedehydrogenase, platelet, leukocytes counts and pH were evaluated. Results: During storage for up to 5 days, no significant pH, difference was observed among all three type of platelet concentrates (p>0.05). The mean leukocytes count buffy coat units, platelet rich plasmaplatelet concentrates and apheresis-derived units were comparable and statistically significant difference was observed (p<0.05). During storage for up to 5days platelet concentrates units displayed significant an increase in lactatedehydrogenase, CD63 and Annexin V expressions, as compared with buffy coat units and apheresisderived units preparation on day 5 (p<0.05). Discussion: The kinetics of CD63 and annexin V levels are influenced by the method used to prepare platelets for storage. The different levels of CD63, annexin V and lactatedehydrogenase in three types of units clearly demonstrating a progressive activation process of platelet concentrates units exceeds that of buffy coat and apheresis-derived units. Further clinical studies will be necessary to determine whether meet the quality criteria or is superior in predicting in vivo viability