AA-Dutp-Cy3 a Novel Fluorescent Labeling Agent for Nucleotides

A bioassay was developed to provide a process for the synthesis of cyanine dye labeled base modified nucleoside triphosphates analogues as a novel labeling agent for biomolecules. It is also to provide a low cost process and more specifically relates to a process for the synthesis of cyanine fluorophore - base modified nucleoside triphosphates analogues as well as for efficient and robust labeling of RNA and cDNA as hybridization probe in microarray detection and analysis