In-silico Structural and Functional Analysis of Hypothetical Proteins of Leptospira Interrogans

Though after a start of genome sequencing most of the protein sequences are deposited in databases, some proteins remain to be annotated and functionally characterized. Analyzing and annotating the function of Hypothetical proteins of Leptospira interrogans is very important which is a pathogenic spirochete causes various complications in human and animals. Randomly we have selected 12 sequences of hypothetical proteins and were analyzed through web tools as Pfam, CD Blast, Expasy's to determine physicochemical properties and protein family information based on conserved domains. Proteins from families AdoMetDC, LRR, and PilZ are most conserved in many microorganisms can be targeted to develop efficacious drug molecules. Ligand binding site and protein structure prediction was done by using Qsite finder and PS2 server which will help to develops therapeutic molecules in docking studies. Present study has shown intracellular interactions of proteins involved in drug resistance, transcription factors, and transport channels.