Study on Black Gram (Vigna mungo L.) Infected with Meloidogyne incognita under the Influence of Pseudomonas fluorescens, Bacillus subtilis and Urea

Pot studies were carried out to assess the growth and biochemical components of Vigna mungo L. cultivar ‘Azad- 2’ with the root-knot nematode and treated with two biofertilizers viz. Pseudomonas fluorescens strain CHA0, and Bacillus subtilis strain Bs-5. The plants were grown in soil at N50 and N100 doses of urea. Uninoculated untreated plants served as control. From the results, it was evident that V. mungo plants exhibited profused vegetative growth at N100 dose of urea as was observed in the treatment 5. The plants of treatment 5 were inoculated with P. fluorescens CHA0 (20 ml). The number of nodules per plant was found increased to 14.33 per plant, total chlorophyll content by 2.91 mg/g of leaf tissue, leghaemoglobin by 3.81 mg/g, and protein content in leaves and seeds by 0.83 and 0.76 mg/g, respectively, when compared with the control and the plants that received different doses of B. subtilis Bs-5 in the absence of the root-knot nematode. Significant reduction in gall number (41.66) and number of nematodes per gram of root (4.66) was recorded in the treatment 5