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Anti-A and Anti-B Haemolysins amongst Group “O” Voluntary Blood Donors in Northeastern NigeriaDOI: 10.4061/2011/302406 Abstract: Background and Objective. The aim of this study was to determine the prevalence and haemolytic significance of alpha- and beta-haemolysins in our voluntary group “O” donor population. Methods. This was a prospective study carried out at North-East Zonal Centre, the National Blood Transfusion Service, Maiduguri, Nigeria from April 2007 to April 2009. One thousand nine hundred and twenty nine voluntary group “O” blood donors (1609 males and 320 females, median age 26 years ± 7.6?SD) were screened for alpha- (anti-A) and beta- (anti-B) haemolysins using the standard tube technique at 37 degrees C for 1 hour. All samples showing haemolysis were titrated for anti-A and anti-B haemolysins. Results. The overall prevalence of haemolysins in group O donors was 55.4%. Prevalence of alpha- and beta-haemolysins only was 10.3% and 12.6%, respectively, while that of donors having both alpha- and beta-haemolysins in their sera was 32.5%. Visual titre of 8 was seen in 0.4% of lytic alpha-haemolysin and 0.2% of lytic beta-haemolysin whereas donors with both alpha- and beta-haemolysins had a titre of 1.8%. Lytic titre of 16 and 32 was very low in our donor population. Conclusion. This study has shown that although the prevalence of haemolysins is high in our voluntary group “O” donor population, the strength of the lytic antibodies is low. Therefore, despite the labour intensiveness of our haemolysis titration technique and the frequent transfusion of group O blood to certain recipients of blood group A, B, and AB in our environments, there is the need to routinely screen our donors for haemolysins in order to identify those posing the greatest risk to recipients. Further studies to determine episodes of clinically significant haemolysis in recipients of blood group O may be necessary. 1. Introduction The importance of a blood group system in clinical blood transfusion practice lies in the frequency of its antibodies and in the possibility that such antibodies will destroy incompatible cells in vivo [1]. Almost everybody over the age of 6 months has clinically significant anti-A and/or anti-B in their serum if they lack the corresponding antigens on their red cells [1]. Blood group “O” red cells can be given to A, B, or AB recipients and were formerly inappropriately called “Universal donor red cells”. Early studies from Nigeria have shown high frequency of potentially lytic anti-A and lytic anti-B in blood group “O” persons [2, 3]. These high frequency of alpha- and beta-haemolysins has been suggested to be responsible for the high frequency of ABO-haemolytic disease of
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