基于GBS、DArT array和SSR标记构建普通小麦高密度遗传图谱
Constructing high density genetic linkage map of bread wheat with GBS,DArT array and SSR markers

以印度小麦品种Sujata 与澳大利亚小麦品系Avocet杂交获得148个家系的重组自交系群体为材料，利用6 397对基于二代测序技术的GBS标记、705对DArT array标记和164对SSR标记构建普通小麦高密度遗传图谱。结果显示：该图谱覆盖小麦的21条染色体，分为25个连锁群，总长度6 104.4 cM，标记间平均距离为0.84 cM。本研究构建的高密度连锁图为后续QTL定位、图位克隆和分子标记辅助选择等研究奠定基础。
Genetic linkage map based on classical molecular marker platforms with low density cannot meet the requirements for wheat functional genomics studies due to its huge and complex genome. 148 recombinant inbred lines (RILs) derived from the crossing Sujata (Indian wheat variety) with Avocet (Australia wheat variety) were used to construct the high density genetic linkage map with 6 397 genotyping by seqencing(GBS) markers,705 DArT array markers and 164 simple sequence repeat (SSR) markers. 21 chromosomes were covered by 25 linkage groups with a total length of 6 104.4 cM. It will provide an important resource for QTL mapping,map based cloning and marker assisted selection in wheat breeding.