亮氨酸对秋繁时黄颡鱼精子活力的影响
Effects of leucine on sperm motility of yellow catfish during periods of autumn breeding

在黄颡鱼（Pelteobagrus fulvidraco)精卵质量较差的秋繁早期，挑选体质量接近100 g的雄性黄颡鱼并分为3组，分别注射生理盐水（对照组）、100 μmol/L亮氨酸（亮氨酸适量处理组）和1 000 μmol/L亮氨酸（亮氨酸高浓度处理组），剂量均为200 μL，研究亮氨酸对秋繁时黄颡鱼精子活力的影响。通过计算机辅助精子分析（CASA）系统检测精子活力，发现亮氨酸适量处理组的精子活力衰退速度减小，精子运动时间提高。将3组黄颡鱼精子用于人工繁殖，发现亮氨酸适量处理组受精率为34.9%±4.7%，相比对照组的15.9%±2.2%有显著性提高；亮氨酸高浓度处理组精子的受精率为16.4%±3.4%，与对照组相比无明显变化（P＜0.05）。结果表明适量的亮氨酸能提高黄颡鱼精子活力，但过量的亮氨酸并不能提高精子的繁殖能力。在黄颡鱼秋繁生产上可以适度添加亮氨酸以提高受精率。
To study the effects of leucine on the sperm motility of yellow catfish (Pelteobagrus fulvidraco) during early stage of autumn breeding when the quality of sperms and eggs is lower than in summer breeding,males about 100 g were divided into three groups and injected with 200 μL physiological saline (control group),100 μmol/L leucine (moderate leucine treatment group) and 1 000 μmol/L leucine (high concentration leucine treatment group),respectively.It was found that sperm motility of the moderate leucine treatment group declined more slowly detected by the computer assisted sperm analysis (CASA) system.In artificial reproduction,the fertilization rate of the moderate leucine treatment group sperm was 34.91%±4.7%,significantly higher than that of the control group (15.9%±2.2%) (P＜0.05),and the fertilization rate of the high concentration leucine treatment group sperm was 16.4%±3.4%,significantly lower than that of the moderate group (P＜0.05),but not significant different from the control group (P＞0.05).The results showed that moderate leucine could enhance the sperm motility of yellow catfish,but excessive leucine could not improve the sperm reproduction.So appropriate leucine can be added to improve the sperm reproduction of yellow catfish during the early stage of autumn breeding.