芥菜抗芜菁花叶病毒种质资源的鉴定与评价
Identification and evaluation of resistance to Turnip mosaic virus in germplasm resources of mustard (Brassica juncea)

对33份不同变种的芥菜种质资源材料(大头芥4份、笋子芥1份、 茎瘤芥3份、分蘖芥6份、大叶芥11份、结球芥2份、长柄芥1份、籽用芥1份、宽柄芥菜4份)进行人工机械摩擦接种，通过病情调查和抽薹期ELISA检测，最终筛选出9份抗病毒病材料。在病情调查中， 17Y 7V、17Y 36V病情指数都为0，表现为免疫，其ELISA鉴定DP/DN比值小于2.1，分别为1.38、1.97，呈阴性； 17Y 13V、17Y 20V、17Y 21V、17Y 31V、17Y 33V、17Y 34V、17Y 35V病情指数分别为2.86、5.33、2.00、8.00、5.93、5.71、2.00，表现为高抗，其ELISA鉴定DP/DN比值分别为1.12、0.89、1.60、0.93、0.78、0.81、1.33，均小于2.1，呈阴性。
Thirty three Brassica materials including four Brassica junceaCoss.var. megarrhiza Tsen et Lee,one B. juncea var. crassicaulis Chen et Yang,three B. juncea var. tumidaTsen et Lee,six Brassica juncea var. multicepsTsen et Lee, eleven Brassica juncea var. rugosa Bailey,two Brassica junceavar.capitata Hort,one Brassica juncea var. longepetiolata Yang et Chen,one Brassica junceaCoss.var. gracilis Tsen et Lee,four Brassica juncea var.latipa Li were inoculated with TuMV isolate.Through disease investigation and convulsion ELISA ,nine mustard materials were found to be TuMV resistant,laying the foundation for disease resistance breeding.The condition indexes of both of the numbers 17Y 7V and 17Y 36V in disease investigation were 0, showing immunity.Its DP/DN ratio of the ELISA test were less than 2.1, which were 1.38 and 1.97,showing negative performance.The condition index of the numbers 17Y 13V, 17Y 20V, 17Y 21V, 17Y 31V, 17Y 33V, 17Y 34V, and 17Y 35V in disease investigation were 2.86, 5.33, 2.00, 8.00, 5.93, 5.71, 2.00, showing high resistance. Its DP/DN ratio of the ELISA test were less than 2.1, which were 1.12, 0.89, 1.60, 0.93, 0.78, 0.81, and 1.33, showing negative performance