Development of an enzyme-linked immunosorbent assay for quantification of estriol in milk

ABSTRACT In this study, a heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of estriol (E3) in milk was developed. Rabbit polyclonal antibody specific for E3 was raised by introducing a carboxyl spacer arm from bromoacetic acid of E3 as immunizing hapten. A heterologous ciELISA using a coating hapten containing a carboxyl spacer arm from 6-ketoestriol of E3. The assay exhibited the required sensitivity for detection of E3 with limit of detection of 0.07？ng mL？1 and a working range from 0.41？ng mL？1 to 15.78？ng mL？1. Good recoveries were obtained ranging from 83.2% to 125.8% for E3 spiked milk samples. A satisfactory correlation (R2？=？0.994) between the results of ciELISA analysis and that of LC–MS/MS analysis indicated good accuracy and reproducibility of the proposed method, which was ideally suited as a rapid screening tool for routine E3 monitoring