敲低EN2诱导肝癌细胞凋亡并提高PTEN蛋白的表达
DOI: 10.3971/j.issn.1000-8578.2019.18.1295
Keywords: 肝癌细胞,EN2,PTEN,凋亡,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,LncRNA MEG3 Suppresses Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating miR-543/PTEN Axis,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma,RNA PolymeraseⅠInhibitor Regulates Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb,Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells,Effect of STAT1 on Cell Cycle of Human Glioma U251 Cells and Related Mechanism,Role of Gadd45 Protein Family in Tumorigenesis and Development
Abstract:
摘要 目的 探讨EN2对肝癌细胞凋亡及细胞中PTEN蛋白表达的影响。方法 用EN2 siRNA慢病毒感染肝癌细胞HuH-7，qRT-PCR和Western blot方法检测干扰效果。MTT方法测定细胞活力变化，PI单染法测定细胞周期分布，Annexin V-FITC/PI双染法测定细胞凋亡变化，Western blot测定细胞中激活型Caspase-3（Cleaved Caspase-3）、激活型Caspase-9（Cleaved Caspase-9）、第10号染色体同源缺失性磷酸酶-张力蛋白（PTEN）及细胞周期素B 1( Cyclin B l)蛋白水平，JC-1法测定线粒体膜电位变化，Western blot测定胞浆中细胞色素C（Cytochrome C）蛋白水平。结果 EN2 siRNA慢病毒感染可明显下调肝癌细胞中EN2的表达。沉默EN2表达后的肝癌细胞活力降低，细胞凋亡率升高，细胞G2/M期比例升高，细胞中Cleaved Caspase-3、Cleaved Caspase-9、PTEN蛋白水平明显升高，Cyclin B1蛋白水平明显降低，线粒体膜电位下降，胞质中Cytochrome C蛋白水平升高。结论 敲低EN2可以阻滞肝癌细胞周期，诱导肝癌细胞凋亡，其作用机制可能与PTEN、线粒体凋亡途径有关
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