前列腺癌移植瘤近红外荧光成像最佳成像时间的实验
DOI: 10.3971/j.issn.1000-8578.2019.18.1900
Keywords: A Retrospective Study,Application of PSMA PET/CT Specific Molecular Imaging in Diagnosis of Non-prostate Cancer,A Comprehensive Review of Androgen Deprivation Therapy and Immunotherapy on Prostate Cancer,Application of Superb Micro-vascular Imaging Technology in Guiding Accurately Targeted Biopsy of Prostate Cancer,Efficacy and Prognostic Factors of Docetaxel Rechallenge on Metastatic Castrationresistant Prostate Cancer Patients,Efficacy and Prognostic Factors of Docetaxel Combined with ADT on Metastatic Hormone-sensitive Prostate Cancer Patients,The Second Primary Cancers in Patients with Prostate Cancer: An Update,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Qualitative Diagnostic Value of Different Apparent Diffusion Coefficients on Metastatic Pelvic Lymph Nodes in Prostate Cancer,Research Progress of Tumor Microenvironment and Prostate Cancer Bone Metastasis,Influence of Periprostatic Adiposity Measurement Parameters on Preoperative Staging and Grading of Prostate Cancer,Effect of Lutein on Proliferation and Apoptosis of Human Prostate Cancer PC3 Cell Line and Related Mechanism,Application of Near-infrared Fluorescent Imaging in Thoracic Surgery,Robotic-assisted Laparoscopic Radical Prostatectomy: Current Situation and Perspectives,Selection and Validation of Polypeptide Fragment Specially Locating on Surface of Highly Metastatic Prostate Cancer Cells
Abstract:
摘要 目的 探讨前列腺癌荷瘤裸鼠皮下移植瘤吲哚菁绿（ICG）体外近红外荧光成像的动态特征、最佳显影时间及成像机制。方法 选取10只SPF级BALB/c-nu雄性裸鼠，构建人前列腺癌细胞系PC3荷瘤裸鼠模型，其中7只裸鼠尾静脉注射10 mg/kg ICG，分别于注射后不同时间行近红外荧光成像，观察移植瘤及全身荧光影像的动态特征，计算肿瘤背景比（TBR）。3只裸鼠同法注射10mg/kg ICG，24 h后剖取肿瘤组织及不同脏器和瘤旁脂肪组织进行体外近红外荧光成像。取肿瘤组织行病理切片，在荧光显微镜下观察肿瘤组织中ICG的分布。结果 7只裸鼠尾静脉注射ICG后肿瘤部位荧光强度随时间的推移逐渐增强，肿瘤周围的背景荧光逐渐减弱，12 h后TBR最佳。3只裸鼠各器官平均灰度值依次为：胃＞大肠＞小肠＞肝脏＞肿瘤＞肾脏＞瘤周脂肪组织。荧光显微镜下可见肿瘤组织有明显的绿色荧光。结论 静脉注射ICG后，ICG能够通过高通透性及滞留效应，在前列腺癌皮下移植瘤中浓聚，注射后12~24 h成像效果最佳
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