胭脂萝卜RsUFGTs的克隆与表达分析
Cloning and expression of RsUFGTs in red radish

为确定参与胭脂萝卜花青苷生物合成的类黄酮糖基转移酶基因（RsUFGTs），从萝卜基因组中筛选出3个候选的RsUFGTs基因，以胭脂萝卜为材料，克隆了3个RsUFGTs的开放阅读框（ORF）并进行序列及表达分析。结果表明：RsUFGT1和RsUFGT2的ORF长度分别为1 383和1 374 bp，分别编码460和457个氨基酸，而RsUFGT3提前终止；氨基酸序列比对和系统进化树分析表明RsUFGT1和RsUFGT2均含有PSPG保守特征结构域，且属于Cluster Ⅰ亚族成员；利用实时荧光定量PCR分析它们在不同着色萝卜品种中的表达发现，RsUFGT1 在‘红心1号’胭脂萝卜皮和肉组织中表达量较高，在‘满堂红’萝卜皮和‘春不老’的萝卜皮与肉中均不表达，其表达量与花青苷含量呈正相关，而RsUFGT2的表达则无明显规律。因此，RsUFGT1可能是胭脂萝卜花青苷生物合成的重要结构基因之一。
To clarify the flavonoid glycosyltransferase gene（RsUFGTs） involved in the biosynthesis of anthocyanin in red radish,three candidate RsUFGTs genes were screened from the radish genome,and the open reading frames (ORFs) of three RsUFGTs were cloned and sequenced and expressed. Results showed that the ORF length of RsUFGT1 and RsUFGT2 was 1 383 bp and 1 374 bp,encoding 460 and 457 amino acids,respectively. However,RsUFGT3 was mutated with early termination. Protein sequence alignment and phylogenetic tree analyses indicated that RsUFGT1 and RsUFGT2 both contain PSPG conserved domain and belong to the same UFGT class. The results of real time quantitative PCR analysis showed that the expression of RsUFGT1 in ‘Hongxin No.1’ was relatively high and no expression was detected in skin of ‘Mantanghong’ and in skin and flesh of ‘Chunbulao’. The expression of RsUFGT1 was positively correlated with anthocyanins content in tissues of different radish cultivars. However,no obvious rules of RsUFGT2 expression were detected. It is indicated that RsUFGT1 may be one of the important structural genes of anthocyanin biosynthesis in red radish.