小鼠STEAP4对HepG2细胞自噬的调控机制
Impact of mice STEAP4 on autophagy in HepG2 cells

为研究机体内稳态调节机制，经筛选高脂小鼠转录组数据得到特异上调的Steap4基因，对其进行不同物种间基因多态性比较，并在HepG2细胞中异源超表达该基因24 h后检测自噬相关基因在转录和翻译水平的变化。结果显示，STEAP4基因在不同物种间较保守，其基因多态性主要在翻译水平产生变化，小鼠Steap4基因与人源STEAP4基因相似性最高。荧光定量PCR的结果表明mSTEAP4对HepG2自噬关键基因无显著性影响，但Western blot结果显示mSTEAP4能够下调自噬相关蛋白的表达量，即mSTEAP4从蛋白水平抑制HepG2细胞自噬水平。STEAP4在细胞处于内稳态时会抑制自噬，降低细胞中的物质能量代谢，使细胞处于静息状态。
In order to study the regulation mechanism of homeostasis in vivo,Steap4 gene,which was upregulated specifically,was obtained by screening the transcriptome data of high fat feeding mice.Polymorphism of STEAP4 among different species was compared,and the changes of autophagy related genes at transcription and translation levels were detected 24 hours after transfection in HepG2 cells.The results showed that STEAP4 was conservative among different species,their gene polymorphism mainly changed at the protein level,and the similarity of STEAP4 between mouse and human was the highest.The results of fluorescence quantitative PCR showed that mSTEAP4 had no significant effect on autophagy genes in HepG2 cells,and the results of Western blot showed that mSTEAP4 could reduce the expression of autophagy related proteins,suggesting that mSTEAP4 inhibits the autophagy at the protein level in HepG2 cells.STEAP4 could inhibit autophagy in homeostasis,reduce material and energy metabolism,and keep cells in the resting state.