外源物质对红松多酚合成和防御酶活性的影响
Effects of Exogenous Substances on Polyphenols Synthesis and Defence Enzymes Activites in Adventitious Buds of Pinus koraiensis

研究3种外源物质（壳聚糖CTS、茉莉酸甲酯MeJA、硝酸镧La）对松多酚合成的诱导作用。以组织培养诱导生成的红松不定芽为试验材料，CTS、MeJA、La处理8 d过程中对不定芽生长、松多酚积累量、多酚合成关键酶（苯丙氨酸转移酶PAL、肉桂酸4-羟化酶C4H）、防御酶（超氧化物歧化酶SOD、过氧化物酶POD、过氧化氢酶CAT）影响。结果表明，CTS、MeJA、La均能促进松多酚的合成，其诱导效果存在差异，以La诱导效果最显著。在试验条件浓度下，La不仅可以促进红松不定芽的生长，且能显著提高总酚和原花青素的积累量。经La处理的红松不定芽中的SOD、POD、CAT、PAL、C4H能够迅速响应，并且活性均呈现先升高后降低的趋势，各酶活峰值分别是对照组的1.79、1.51、1.33、3.98倍和3.61倍。CTS、MeJA、La 3种外源物质能激活红松防御反应，促进次生代谢产物松多酚的合成。
The objectives of this study were to investigate the induction of pine polyphenols biosynthesis by three exogenous substances including chitosan (CTS),methyl jasmonate (MeJA) and lanthanum nitrate (La).Adventitious bud of Pinus koraiensis was used as the material,the effects of three exogenous substances were examined on the growth,the pine polyphenols accumulation,the key biosynthesis enzymes and the defense enzymes.The results showed that CTS,MeJA and La had the abilities to facilitate the syntheses of pine polyphenol with different efficiencies,in which La presented the most significant effects.In the condition of examination concentration,La was able not only to facilitate a growth of adventitious bud,but also to increase the accumulations of total polyphenol and proanthocyanidin.SOD,POD,CAT,PAL,C4H in the adventitious bud responded the La treatment rapidly.The activities of SOD,POD,CAT,PAL,C4H increased first,and then decreased.The peak values of the corresponding enzymes were 1.79,1.51,1.33,3.98,and 3.61 times more than the control.CTS,MeJA and La could act as potent elicitors to induce the defense responses to stimulate secondary metabolite production in P.koraiensis