白花泡桐和毛泡桐丛枝病发生与蛋白质表达谱变化
Changes of Protein Profile and the Occurrence of Witches’ Broom in Paulownia fortunei and Paulownia tomentosa

为深入了解泡桐丛枝病发生与蛋白质变化的关系，以白花泡桐和毛泡桐健康、患丛枝病组培幼苗以及经15 mg？L-1和75 mg？L-1硫酸二甲酯（DMS）处理的患病组培幼苗为材料，采用iTRAQ技术研究这2个品种、8种材料的蛋白质表达谱变化。结果表明，12种蛋白质与泡桐丛枝病发生密切相关，其主要定位于叶绿体（7个，58.33%）和线粒体（3个，25%），功能与光合作用、蛋白质翻译、抗逆性相关。实时荧光定量PCR验证结果显示部分蛋白质的基因在mRNA的表达水平与iTRAQ测试的蛋白质翻译水平结果一致。研究结果为深入揭示泡桐丛枝病发生机理奠定基础。
In order to understand the relationship between the occurrence of paulownia witches’ broom and the protein profile changes of the host,in this study,the technology of isobaric tags for relative and absolute quantitation (iTRAQ) was adopted to analyze the protein changes in the healthy,infected,and 15 mg？L-1,75 mg？L-1 dimethyl sulfate treated infected tissue culture plantlets of two Paulownia species (P.fortunei and P.tomentosa).A total of 12 differentially abundant proteins closely related to the witches’ broom were obtained.Subcellular localization showed that 7 proteins were located in chloroplasts and 3 located in mitochondria,with functions related to photosynthesis,protein translation,and stress resistance.Then the quantitative real-time PCR (qRT-PCR) showed the expression level of the differentially abundant proteins coding genes were consistent with the generated by iTRAQ results.The results would lay the foundation for revealing the mechanism of paulownia witches’ broom