不同红蓝光质比对大花萱草组培苗增殖及生根的影响
Effects of Different Red and Blue Light Quality Ratios on Multiplication and Rooting of Hemerocallis middendorfii Plantlets in vitro

以大花萱草组培苗为材料，研究不同红蓝光质比LED对组织培养过程中增殖及生根的影响。结果表明，红光更利于大花萱草丛生苗生物量和可溶性糖以及生根苗株高和生物量的增加，但不利于大花萱草色素和可溶性蛋白的积累；蓝光有利于大花萱草色素和可溶性蛋白的合成，但不利于生根苗叶数、根数和生物量的增加；红蓝组合光有助于提高丛生苗增殖系数、生根苗叶数和根系活力，并能够促进大花萱草可溶性糖和可溶性蛋白的合成和积累。综合考虑各项指标，大花萱草增殖阶段的最适光质为红蓝光质比1∶1，大花萱草生根阶段的最适光质为红蓝光质比6∶4，该结果为LED光质在大花萱草组织培养中的应用奠定基础。
Hemerocallis middendorfii plantlets were used as materials to investigate the effects of different red and blue light quality ratios on multiplication and rooting in vitro.The results showed that red light was more conducive to the increase of biomass and soluble sugar of shoots and the height and biomass of rooting seedlings,but it was not conducive to the accumulation of pigment and soluble protein.Blue light was beneficial to the synthesis of the pigment and soluble protein,but it was not conducive to the increase of leaf number,root number and biomass of rooting shoots.The combination of red and blue light was favorable to increase the multiplication coefficient of the shoots,the number of rooted shoots,and the root activity,and could promote the synthesis and accumulation of soluble sugar and soluble protein.Considering each index comprehensively,the optimum light quality in the multiplication stage was the combination of red to blue light with a ratio of 1∶1,in the rooting stage,the ratio was 6∶4.The results would lay a foundation for the application of LED light quality in tissue culture of H.middendorfii