益母草活性成分芫花素对小鼠凝血及抗炎作用的研究
Pharmacological effects of the active ingredient genkwanin in Leonurus on coagulation and anti-inflammation in mice

摘要：目的 研究益母草中的活性物质芫花素对小鼠凝血及抗炎的药理作用。方法 采用毛细玻管法及玻片法考察芫花素对小鼠凝血功能的影响；采用醋酸致小鼠疼痛扭体、二甲苯致小鼠耳廓肿胀及血管通透性模型研究芫花素的抗炎镇痛作用。结果 芫花素中剂量、大剂量组小鼠凝血时间均较空白对照组明显缩短(P<0.05)；芫花素中剂量组和模型对照组相比，小鼠凝血时间显著缩短(P<0.05)；芫花素大剂量组与模型对照组相比较，扭体次数显著减少(P<0.01)。芫花素对二甲苯致小鼠耳廓肿胀的影响结果表明，阳性对照阿司匹林组与模型对照组比较，左右耳片重差值差异有统计学意义(P<0.01)，芫花素大剂量组与模型对照组相比差异有统计学意义(P<0.05)。在芫花素对小鼠腹腔毛细血管通透性影响的试验中， 阳性对照阿司匹林组与模型对照组比较，A值减小(P<0.01)；芫花素小剂量组与模型对照组相比，A值无明显改变(P>0.05)；芫花素中剂量组与模型对照组比较，A值显著减小(P<0.05)；芫花素大剂量组较模型对照组A值显著减小(P<0.01)。结论 芫花素可促进小鼠凝血功能；减少醋酸致小鼠扭体疼痛次数，有一定的镇痛作用；抑制二甲苯致小鼠耳廓肿胀及降低小鼠腹腔毛细血管通透性，具有一定的抗炎作用。这为其治疗痛经及子宫内膜炎等疾病提供了临床依据。
ABSTRACT: Objective To investigate the pharmacological effects of genkwanin, the active ingredient in Leonurus on coagulation and anti-inflammation in mice. Methods The body torsion pain caused by acetic acid model, auricle swelling induced by xylene, and the blood capillary permeability model were used to investigate the analgesic and anti-inflammatory effects of genkwanin in mice. Results The clotting time was significantly reduced in medium- and high-dose genkwanin groups compared with the control group (P<0.05). The times of mice’s body torsion pain caused by acetic acid was significantly reduced at 10 minutes after administration in the large-dose group compared with the control group (P<0.01). Compared with the model group, the positive control (aspirin) group had a significant difference (P<0.01) in the difference value of left- and right-ear weights, the high-dose group had significant difference (P<0.05). In the experiment on the influence of genkwanin on blood capillary permeability of the mice abdominal cavity, compared with the model group, a marked decrease of A value (P<0.01) was found in the positive control group, but there was no significant difference of A value (P>0.05) in the low-dose genkwanin group, a significant reduce of A value (P<0.05) in the medium-dose group, and an extremely significant decrease of A value (P<0.01) in the high-dose group. Conclusion Genkwanin can promote blood coagulation function of mice, and reduce the times of their body torsion pain caused by acetic acid, showing obvious analgesic effects. Furthermore, genkwanin can inhibit auricle swelling of mice induced by xylene and reduce the blood capillary permeability of their abdominal cavity, suggesting its mild anti-inflammatory effects. The findings can provide clinical evidence for treating dysmenorrheal and endometritis