雷公藤红素对胃癌细胞增殖及有氧糖酵解的影响
Effects of celastrol on proliferation and aerobic glycolysis of gastric cancer cells

摘要：目的 探究雷公藤红素对胃癌细胞BGC-823增殖及有氧糖酵解的影响。方法 BGC-823细胞经雷公藤红素处理后，CCK8实验与克隆形成实验检测细胞增殖能力；流式细胞术检测细胞凋亡与周期；分光光度计法检测细胞葡萄糖消耗量、乳酸生成量及己糖激酶(HK)、乳酸脱氢酶(LDH)活性变化；Western blot检测 BGC-823细胞内葡萄糖转运体1(GLUT1)、己糖激酶Ⅱ亚型(HKⅡ)、丙酮酸激酶M2亚型(PKM2)、LDH蛋白表达。结果 雷公藤红素作用后，BGC-823细胞增殖受到抑制，细胞凋亡增加，细胞周期阻滞于G2/M期。BGC-823细胞葡萄糖利用量及乳酸生成量降低，GLUT1、HKⅡ及LDH蛋白表达下降，HK、LDH酶活性受抑制。结论 雷公藤红素抑制BGC-823细胞增殖，其作用可能与抑制细胞有氧糖酵解有关。
ABSTRACT: Objective To explore the effects of celastrol on the proliferation and aerobic glycolysis of gastric cancer cell line BGC-823. Methods After treatment with celastrol, the proliferation of BGC-823 cells was detected by CCK8 assay and clone formation assay; cell apoptosis and cell cycle distribution were detected by flow cytometry. Glucose utilization, lactic acid production, hexokinase (HK) activity and lactate dehydrogenase (LDH) activity were detected by spectorphotometry; Western blot was conducted to detect the expressions of glucose transporter 1(GLUT1), hexokinase Ⅱ (HKⅡ), pyruvate kinase M2 (PKM2), and LDH in BGC-823 cells. Results Exposed to celastrol, BGC-823 cells’ proliferation was inhibited, cell apoptosis was induced, and cell cycle was blocked in G2 phase. Glucose utilization and lactate production were decreased. Moreover, celastrol downregulated the expressions of GLUT1, HKII, and LDH protein as well as the activities of HK and LDH enzyme. Conclusion Celastrol can inhibit the proliferation of BGC-823 cells and it may be related to the inhibition of aerobic glycolysis