In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (portulacaceae) - In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (portulacaceae) - Open Access Pub

In this study, the antioxidants and photosynthetic compounds of Verdolaga were examined. Compounds were extracted from distinctive segments of the verdolaga using various solvents such as methanol (40, 60, 80%), ethanol (40, 60, 80%), acetone (40, 60, 80%), and deionized water. The use of 80% methanol led to the highest extracted concentration of phenolic substances and flavonoids. The extracted products (Leaves, Stem strips, and Root strips) were evaluated for their radical scavenging capabilities with DPPH (IC50= 22.26, 20.56, and 32.10), and ABTS (IC50= 2.86, 3.70, and 5.24), reducing power (EC50= 15.70, 16.39, and 21.69), and peroxide scavenging activity (1C50= 1.717, 2.937, and 3.255), respectively. The extracted products were analyzed by a gas chromatography-mass spectrometer. Peroxidase, catalase, and polyphenol oxidase assays were completed for the crude extract of verdolaga’s leave, stem strips, and root strips. As indicated by these tests, extracts of the verdolaga’s roots, stems and leaves using 80% methanol yielded high antioxidant activity. The most elevated concentrations of extracted chlorophyll, lycopene, and carotenoids were from the leaves and the highest concentration of extracted tannin was noted from strips of stems. The highest measures of peroxidase and polyphenol oxidase were identified in root strips and the highest units of catalase was identified in leaves. DOI10.14302/issn.2379-7835.ijn-19-3144 Reactive oxygen species (ROS) are produced in higher volumes during tissue injury. An excessive amount of ROS can denature deoxyribonucleic acid (DNA) and proteins, disrupt cell layers, and negatively affect lipids through chain reactions 1. The production of reactive oxygen species (ROS) mediated lipid peroxidation plays a key role in cell death, including autophagy, ferroptosis, and apoptosis. Cell reinforcements, such as atoms that have the capability to neutralize radical, protect against these damages. These compounds can assist in forestalling diseases, including malignancy, hepatitis, asthma, atherosclerosis, joint inflammation, coronary illness, and diabetes 2. Recently, plants and herbs have been placed in dietary supplements as cancer prevention agents, as natural alternatives to manufactured cell reinforcements 3. The reports of side effects from synthetic ingredients of supplements have prompted buyers to explore options that are associated with less egregious results 4. It has been reported that extracts from herbs are able to support cell defense mechanisms and stimulate antimicrobial activity 3, 5. The extraction of