Development and Validation for Simultaneous Estimation of Proguanil and Atovaquone by using RP-HPLC

A simple, precise and rapid RP-HPLC method has been developed for the simultaneous determination of Proguanil and Atovaquone in pharmaceutical dosage form. The method was carried out using Kromasil C18 column (150 mm x 4.6 mm, 5 μm) and mobile phase comprised of 0.1% Ortho Phosphoric Acid(OPA):Acetonitrile(ACN) in the ratio of 50:50 v/v and degassed under ultrasonication. The flow rate was 1.0 mL/min and the effluent was monitored at 287 nm. The retention times of Proguanil and Atovaquone were 2.15 min and 2.48 min respectively. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of detection and limit of Quantitation. Linearity was in the range of 25 to 150 μg/mL for Proguanil and 62.5 to 375 μg/mL for Atovaquone respectively. The percentage recoveries of both the drugs were ranging from 98.86 to 99.97% for Proguanil and 99.52 to 99.79% for Atovaquone respectively from the tablet formulation. The proposed method is suitable for the routine quality control analysis of simultaneous determination of Proguanil and Atovaquone in bulk and pharmaceutical dosage form. Keywords: Proguanil; Atovaquone; RP-HPLC; Validatio