Intrageneric Phylogenetics based on Available Three Mitochondrial Genes and One Nuclear Gene Variation among Thirteen Species of Coranus Curtis and Two Ecotypes (Hemiptera: Reduviidae: Harpactorinae)

The sequence of three mitochondrial genes viz., 16S rRNA, Cyt b, COI and one nuclear gene, 28S rRNA of Coranus Curtis species downloaded from the GenBank were subjected to phylogenetic analyses to understand the intrageneric and intraspecific variations and the role of geographical isolation on speciation using CLUSTAL W in MEGA version 5.6. This analysis includes thirteen species of Coranus Curtis and probably two ecotypes of Coranus callosus St？l from four countries viz., Australia, Brunei, China and Nigeria of three continents viz., Africa, Asia and Australia. The pairwise genetic distances were calculated and phylograms were constructed using maximum likelihood, neighbor-joining, minimum evolution, UPGMA and maximum parsimony methods. These preliminary analyses not only demarcated the thirteen species of Coranus and the two ecotypes of C. callosus but also revealed phylogenetic relationships and the role of geographical isolation on speciation. Keywords: Coranus; 16S rRNA; Cyt b; COI and 28S rRNA; Harpactorinae; Biocontrol Agents; Intrageneric Molecular Biosystematics; Speciation; Ecotypes; Geographical Isolation