Using Immunofluorescence to Investigate an Associated Specific Humoral Immune Response Role in Climatic Droplet Keratopathy

Purpose: To find out any associated specific immune role in Climatic Droplet Keratopathy (CDK) through immune fluorescent study and electron microscopy that can provide a new therapeutic strategy in the prevention and in the progression of the disease. Methods: A total of 22 case of CDK who underwent keratectomy or scraping were incorporated in this study. Twenty of them were subjected to immuno-fluorescent study using IgG, IgM, IgA and complement 3 and the remaining two cases were subjected to electron microscopic study. Results: Although, the electron microscopic study showed extracellular electron dense like globules in the superficial corneal stroma just beneath the epithelium that may stimulate the immune complex humps of immune-mediated glomerulonephritis, the immuno fluorescent examination for all the studied cases revealed total negative stain for all studied markers (IgG, IgM, IgA and C3). Conclusion: This work suggests the absence of specific immune response in climatic droplet keratopathy. Keywords: Climatic droplet keratopathy, immune complex, immuno fluorescent