Investigation Of Nitric Oxide Synthase 3 (Nos3) Glu298asp Gene Variation In Breast Cancer Patients

The role of Nitric Oxide (NO) in tumor biology is not well understood. However, it has been proposed that NO is a highly reactive free radical and plays important roles in growth, progression or metastasis of tumour. Nitric oxide syntheses (NOSs) are a family of enzymes that catalyzing the production of NO by converting L-arginine to L-citrulline. The aim of present study was to examine Nitric Oxide Synthase 3 (NOS3) Glu298Asp polymorphism in patients with breast cancer. For this purpose, 49 patients with breast cancer and 89 healthy controls were included in the study. The NOS3 Glu298Asp polymorphism was genotyped by PCR-RFLP using peripheral blood samples in our study groups. NOS3 Glu298Asp genotype distribution in the breast cancer patients was statistically different from the healthy control group NOS3 Glu298Asp gene variation T allele frequency was significantly higher in breast cancer patients that of controls. Our finding suggests that NOS3 Glu298Asp might be associated with breast cancer risk. Key words : Oxidative stress; NOS3; breast cancer