Cytotoxic effects of steroidal glycosides isolated from the Paris vietnamensis plant on cancer cell lines and against bacterial strains

Abstract Extracts from the rhizomes of Paris species have been used in Asia to help treat inflammation, infection, analgesia, detoxification, dysentery, etc. Analysis of the chemical components from the rhizomes of Paris vietnamensis showed that they contained polyphenols, flavons, flavonols, flavonoids, tannins and steroidal and triterpenoid saponins. In this study, P. vietnamensis was identified from the sample collected from Sapa, Laocai province, Vietnam and three steroidal glycosides from rhizomes of this species were evaluated for anticancer and antimicrobial activity in vitro. The samples were identified as belonging to P. vietnamensis species through a combination of morphological characteristics and DNA barcodes based on the nucleotide sequence of the ITS and matK. All three steroidal glycosides exhibited strong cytotoxic activity against SK-LU-1, HeLa and MKN7 cell lines with IC50 values ranging from 1.07 to 4.37？μmol L？1, and exhibited high antimicrobial activity against strains of Serratia marcescens, Escherichia coli, Lactobacillus plantarum and Bacillus subtilis. This study provides information on the antibacterial and inhibitory activities of steroidal glycosides derived from the rhizomes of P. vietnamensis collected from Sapa, Laocai province, Vietnam on cancer cell lines. Thus, P. vietnamensis could be used as an alternative to other species of the Paris genus for anticancer and antimicrobial treatment