AGRP neurons modulate fasting-induced anxiolytic effects

Elevated plus maze experiments were performed in both male and female mice in the ELP and a cohort study in the LLP. Representative traces of plus maze experiments on male mice respectively in the ELP (a) and the LLP (b). c Grouped data of average time spent in the open arms from a and b experiments. d Grouped data of open arms distance. e–h. Mice were tested by using open field behavioral assays in the ELP and a cohort study in the LLP. Representative traces of open field tests in the ELP (e) and in the LLP (f) (n？=？5 male). g Grouped data of percent time spent in the center of the open field. h Grouped data of total distance traveled during the whole eperiments. Two-way ANOVA with Sidak’s post hoc tests were performed for c, d; Unpaired Student’s t-test for (g, h). Data represent mean？±？s.e.m. *p？<？0.05; ** p？<？0.01; ***p？<？0.001; n.s. not significan