iPSC model of CHRFAM7A effect on α7 nicotinic acetylcholine receptor function in the human context

a Live images of the UB068 (0 copy) and UB052 (1 copy of CHRFAM7A) colonies stained with TRA1–60 antibody. b Reverse transcription–quantitative polymerase chain reaction analysis of self-renewal/pluripotency gene expression in iPSC lines in comparison with H9 cell line. c Confocal images of the iPSCs probed for Nanog, Oct4, Sox2, and SSEA-4 proteins. Gene (d) and protein (e) expression of the three germ layer markers at day 15 of non-directed differentiation in UB068 and UB052 line