Host-antibody inductivity of virulent Entamoeba histolytica and non-virulent Entamoeba moshkovskii in a mouse model

Mouse antibody response profiles to E. histolytica and/or E. moshkovskii trophozoites. Mice were immunized with trophozoite cells of E. histolytica and/or E. moshkovskii. Level of E. histolytica and E. moshkovskii total antibodies (a, b), species-specific IgG (c, d) and IgA (e, f) were measured by ELISA using goat anti-mouse IgG or IgA labeled with HRP as antibody tracker. B1–B4 represent numbers of serum collected 2 weeks after each 1st-4th immunization and B5 represents serum collected 8 weeks after the 4th boost. The results were presented as means of optical density (OD)？±？standard error of the mean (SEM) from three independent experiment