Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR

Operational scheme of qIPCR. a McAb is more efficiently coated on the inner surface of PCR tube after pretreating with glutaraldehyde; b probe complex is prepared in advance through linkage of streptavidin, biotin-McAb and biotin-DNA. The optimal molar mass ratio is 1:1:1 for biotin-McAb, avidin and biotin-DNA; c ABA sample and prepared probe complex are added into the PCR tube coated with McAb to crosslink. The trapped DNA represents the amount in ABA sample after washing PCR tube to remove the excessive probe complex. The trapped DNA can be quantified by RT-PC