Notch3 inhibits epithelial–mesenchymal transition in breast cancer via a novel mechanism, upregulation of GATA-3 expression

a Expression of Notch3, GATA-3, ERα, E-cadherin, and vimentin analyzed by western blot in breast cancer cell lines. b–d Expression of Notch3, GATA-3, and ERα mRNA analyzed by reverse transcription (RT)–PCR in breast cancer cell lines. e Confocal fluorescence microscopy of DAPI/Notch3/GATA-3 and DAPI/Notch3/ERα staining in MCF-7 (top) and T47D (bottom) cells. The scale bar represents 200？μ