Insights into maize genome editing via CRISPR/Cas9

Representative model depicting CRISPR/Cas9 system for genome modification. The Cas9 protein contains two catalytic nuclease domains: RuvC and HNH. It generates a double stranded break (DSB) at target sites with complementarity to single guide RNA (sgRNA) which can later be edited via Non homologous end—joining (NHEJ) or Homologous—directed repair (HDR