RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity

a Endogenous RecQL4 was detected in anti-Flag pull-down against Flag-AURKB. Flag-GFP immnoprecipitate was used as control. b Endogenous AURKB was detected in anti-Flag-RecQL4 immnoprecipitate determined by Western blotting (lane 2 of Flag IP), and only N-terminus of RecQL4 (Flag-RecQL4-NT, lane 3 of Flag IP) showed an interaction with AURKB. Flag-GFP immunoprecipitate was used for negative control. c Direct interaction between RecQL4 and AURKB was shown by in vitro GST pull-down assa