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ISSN: 2333-9721
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-  2017 

Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize

DOI: 10.1186/s13007-016-0152-4

Keywords: Internal transcribed spacer (ITS), Internal transcribed spacer 2 (ITS2), DNA extraction

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Abstract:

The world’s top three cereals, based on their monetary value, are rice, wheat, and corn. In cereal crops, DNA extraction is difficult owing to rigid non-cellulose components in the cell wall of leaves and high starch and protein content in grains. The advanced techniques in molecular biology require pure and quick extraction of DNA. The majority of existing DNA extraction methods rely on long incubation and multiple precipitations or commercially available kits to produce contaminant-free high molecular weight DNA

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