The transcription factor CREM drives an inflammatory phenotype of T cells in oligoarticular juvenile idiopathic arthritis

CREM is overexpressed in JIA T cells. a Percentages of CREM high expressing T cells of 8 healthy control PBMCs and of 11 oligoarticular JIA SFMCs as assessed by flow cytometry. b Representative histogram showing mean fluorescent intensity of CREM expression in CD3+ T cells. c-j HC PBMCs were treated with 10% SF or HC Serum in RPMI for 24 h. c Percentages of Foxp3+ cells within CD3+ T cells. d Representative dot plot of Foxp3 expressing T cells. e Foxp3 mRNA expression. f Representative dot plot of IL-17+ expressing T cells after restimulation with P/I in the presence of Brefeldin A. g Percentages of IL-17+ cells within CD3+ T cells after restimulation with P/I in the presence of Brefeldin A. h IL-17 mRNA expression. i Geometric (G)-Mean of CREM expression, paired, two tailed t-test. j CREM mRNA expression, two-tailed Mann Whitney test. Symbols present individual patients, SFs or HC sera and horizontal bars show SEM. A two-tailed unpaired t-test was used to calculate p-values in a, b, e, g and