Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells

Phosphofructokinase-1 (PFK-1) is involved in regulating neurogenesis from embryonic neural stem cells (NSCs). A Representative images showing GFP expression in neurospheres infected with LV-Control-shRNA or LV-PFK-1-shRNA (scale bar 200 μm). B, E Immunoblots (B) and statistics (E) showing PFK-1 levels in NSCs infected by LV-Control-shRNA or LV-PFK-1-shRNA. C, D Immunoblots showing the expression of PFK-1-GFP in NSCs infected with LV-GFP or LV-PFK-1-GFP using GFP antibody (C) and PFK-1 antibody (D). F PFK-1 levels in NSCs infected by LV-GFP or LV-PFK-1-GFP. G Schematic of the experimental design for (H–M). Images (H) and statistics (J) of the effect of PFK-1 knockdown on neurogenesis. β-III-tubulin (red), neuronal marker; nuclei were counterstained with Hoechst (blue) (scale bar 100 μm). Images (I) and statistics (K) of the effect of PFK-1 overexpression on neurogenesis. β-III-tubulin (red); Hoechst (blue) (scale bar 100 μm). Images (L) and statistics (M) of the effect of PFK-1 knockdown on the stem-cell-like properties of NSCs. Nestin (red), neural stem/progenitor cell marker; Hoechst (blue) (scale bar 100 μm). Data are mean ± SEM, n = 4, *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed t test)