A functional CRISPR/Cas9 screen identifies kinases that modulate FGFR inhibitor response in gastric cancer

a Schematic overview of the experimental workflow. The whole procedure was repeated to generate two independent biological replicates. b, c Essential kinases identified by the knockout screen. Significant depleted (b) and enriched (c) kinases (FDR？<？0.05) upon FGFR inhibition were labeled in red and blue, respectively. MAGeCK generates both negatively and positively selected false discovery rate (FDR) scores for each gene. Log FC: Log2 fold change. d The summary of essential kinases. Negatively enriched genes were assigned with values generated from negative selection and vice versa for positively enriched genes. RRA: robust ranking aggregation. e Top KEGG pathways identified by gene set enrichment analysis (GSEA) of the 20 essential kinase