LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

SGC7901 (a) or BGC823 (b) cells were treated with pGPU6/GFP/Neo containing shRNA to NC sequences, to LGR5 targeting sequence or with pReceiver-M45-LGR5 or pReceiver-M45 as a control. Expression of LGR5 was assessed by western blot (right panels) 72？h after transfection. The band densities were measured by NIH Image J (left panels). The expression levels of LGR5 in parental SGC7901 and BGC823 were considered as “1