Epitope targeting with self-assembled peptide vaccines

P8 peptide forms a heat stable trimeric coiled-coil. a Circular dichroism spectra: P8 was dissolved in phosphate-buffered saline and subjected to increasing temperature from 5？°C to 95？°C, followed by a return to 5？°C (gray line). b Analytical ultracentrifugation spectrum of P8 showing monomer (0.76？S), trimer (1.78？S), and higher-order assemblies (>3？S). c Intensity-weighted dynamic light scattering spectrum showing major self-assembly (7？nm) and two minor assemblies (25 and 350？nm