Predicting ligand-dependent tumors from multi-dimensional signaling features

Proliferation screen across 58 cell lines. a Ligand/Receptor and antagonistic antibodies used in the in vitro proliferation screen. b Results of the proliferation screen across 58 cell lines. Dots mark a significant increase in ligand induced proliferation or decrease in the presence of ligand plus antibody. The ligand effect is normalized to the medium control, whereas the antibody plus ligand effect is relative to ligand alone. The two cell lines marked with an arrow, as well as five additional cell lines that were not included in the proliferation screen, were used to train the computational model to signaling data. c Correlation pattern of ligand and antibody effects across all cell lines. d Linear correlation of receptor expression to ligand induced proliferation. The proliferation in response to ligand (y-axis) is displayed as log10-fold change with respect to day 0. The receptor surface levels (x-axis) are absolute measurements of receptors/cell by qFACS on a log10-scal