Glutamine synthetase is necessary for sarcoma adaptation to glutamine deprivation and tumor growth

Cellular proliferation was monitored in an IncuCyte FLR. Cell lines were assayed in RPMI 1640 media supplemented with 10% dialyzed FBS and the indicated concentrations of glucose and glutamine. Control (11？mM glucose, 2？mM glutamine), Low Gluc (low glucose, 1.1？mM), No Gluc (no glucose), Low Gln (low glutamine, 0.2？mM), No Gln (no glutamine), and No Gluc/Gln (no glucose/glutamine). Data represent the mean？±？SD of a representative experimen