PTK6 regulates growth and survival of endocrine therapy-resistant ER+ breast cancer cells

PTK6 expression is associated with poor prognosis and promotes growth of ER+ breast cancer cells. a Higher relative PTK6 transcript levels are associated with reduced survival in patients with ER+ breast cancer in the TCGA RNAseqV2 cohort. “High” and “low” PTK6-expressing groups of patients are defined as the top (n？=？207) and bottom (n？=？207) tertiles, respectively, based on log2-transformed PTK6 expression. b, c MCF-7 or T47D cells stably expressing either vector control or activated, membrane-targeted PTK6 (MF-PTK6) were cultured in complete media or in the presence of 4-OHT (1？μM for MCF-7 or 100？nM for T47D cells). Cells were re-fed with 4-OHT containing media every 2–3 days and the cell numbers were counted. Figures are representative of three independent experiments. Error bars are standard error of the mean (s.e.m.). All the western blot images were processed in paralle