MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2

a The certified result of microarray analysis. Hierarchical clustering of seven significantly dysregulated miRNA expression profiles in human primary cervical cancer tissues derived from cervical cancer patients with or without lymph node metastasis. b Validation of the selected miRNAs predicted to be dysregulated in cervical cancer with or without lymph node metastasis using qRT-PCR in the same tissues used for microarray analysis. Data are shown from three independent experiments and presented as fold expression normalized to U6？±？SD (standard deviation). c qRT-PCR analysis of the relative tissue expression of miR-221-3p, miR-135b-5p, miR-25-3p, and miR-144-3p in additional 28 (LNM-N？=？14; LNM-P？=？14) cases of human CC tissues. Each sample was analyzed in triplicate and normalized to U6. *p？<？0.0