MicroRNA-105 is involved in TNF-α-related tumor microenvironment enhanced colorectal cancer progression

a Line chart showing the relationship between TNF-α-NF-κB targeted genes and CRC progression. Twenty representative genes were chosen for line chart plotting. b The morphology of cultured HCT116 cells in response to TNF-α for different periods of time (0–48？h) was observed under an inverted microscope. c Transwell assay on PBS and recombinant human TNF-α treated SW480 cells. The cells were counted under a microscope in five randomly selected fields. Bars of below represent the number of invaded cells (mean？±？SD, n？=？3). d Western blotting analysis was performed to detect EMT markers in both SW480 and HCT116 cells after TNF-α treatment for 24？h and 48？h (a representative set of data was shown, n？=？5