Interference with lactate metabolism by mmu-miR-320-3p via negatively regulating GLUT3 signaling in mouse Sertoli cells

a Localization of mmu-miR-320-3p in the mouse testis was revealed by chromogenic in situ hybridization (CISH). Hybridization signals were in red (Fast-Red), and the nuclei were counterstained with Gill’s Hematoxylin (blue). Specific mmu-miR-320-3p hybridization signals (arrows) were exclusively detected in SCs, whereas the interstitium (IS) and germ cells (GCs) were both negative. Bar？=？25？μm. b Effects of busulfan treatment on testicular histology were assayed using routine hematoxylin and eosin (H&E) staining. Bar？=？20？μm. c Adult male mice were treated with busulfan as described in Materials and methods section. At post-busulfan d 14 and d 30, mice were euthanized, and testes were harvested and subjected to RT-qPCR analysis of mmu-miR-320-3p expression. Different superscript letters denote groups that are statistically different (P？<？0.05). d RT-qPCR analysis of mmu-miR-320-3p expression in primary GCs and SCs isolated from normal mouse testis. Different superscript letters denote groups that are statistically different (P？<？0.05). e RT-qPCR analysis of mmu-miR-320-3p expression in primary SCs isolated from busulfan-treated and Ctrl mouse testis. Different superscript letters denote groups that are statistically different (P？<？0.05). (f) Diagram of the stages of murine spermatogenesis during postnatal development. g qPCR analyses of expression levels of mmu-miR-320-3p during postnatal testicular development. Different superscript letters denote groups that are statistically different (P？<？0.05