MiR-361-3p regulates ERK1/2-induced EMT via DUSP2 mRNA degradation in pancreatic ductal adenocarcinoma

a Association analyses of miR-361b-3p expression levels and TNM stage. b Representative images of the ISH staining analyses of different stage PDAC tissues using anti-miR-361-3p probe. c Association between postoperative overall survival (OS) and tumor miR-361b-3p expression levels in 91 PDAC tissues. The low and high levels of miR-361-3p expression are separated according to the median value. d Comparison of miR-361-3p expression levels in Bxpc-3, Panc-1, CFPAC, SW1990, and HPDE cell lines. e-h Transwell migration/invasion assay and wound healing assay were performed to test the effect of miR-361-3p on PDAC cells metastasis in vitro. i Representative images of orthotopic xenograft pancreatic cancer mouse models from two groups (left panel) and metastatic nodes were calculated (right panel), black arrow indicates primary tumor and red arrows indicate metastatic lesions. j Representative images of liver metastasis model (left panel) and visible metastatic nodes were calculated (right panel), red arrows indicate metastatic lesions. The statistical significance between different groups was calculated with Student t test. Data are shown as the mean？±？SD of three replicates; **P？<？0.01; ***P？<？0.001; ns not significan