Glycogen synthase kinase-3β inhibition promotes lysosome-dependent degradation of c-FLIPL in hepatocellular carcinoma

a The whole-cell protein lysates from six different human HCC cell lines (Huh7, BEL7402, Hep3B, SMMC7721, HepG2, MHCC97H) and one normal hepatocyte line HL7702 were analyzed by immunoblotting (IB) with antibodies against GSK-3β and phospho-GS (p-GS), β-actin was used as the internal control. b The β-catenin levels in whole-cell protein lysates of the seven indicated cell lines were determined by IB. c HL7702, BEL7402, Hep3B, SMMC7721, HepG2, and MHCC97H cells were planted in 6-well plates, the formed clones was showed by hematoxylin dye after 15 days. d Expressions of GSK-3β and p-GS were analyzed by IB in HCC tumor tissues (T) and paired non-tumorous tissues (NT