Genetic modification to induce CXCR2 overexpression in mesenchymal stem cells enhances treatment benefits in radiation-induced oral mucositis

a The mRNA expression levels of various chemokines involved in radiation-induced tongue mucositis were analyzed by RT-qPCR. Samples were extracted from normal and inflamed tongues. The fold change represents the expression of each chemokine mRNA in inflamed tongues compared with normal tongues on day 7 after radiation. All the data are presented as the means？±？standard errors of the means (SEMs) (n？=？6) for each group. b Immunofluorescence staining of CXCL2 (red) in normal and inflamed tongues on day 5 after radiation. The experiments were repeated three times in individual mice. Nuclei were visualized through DAPI staining (blue). Scale bar？=？100？μm. c Total tissue lysates of normal and inflamed tongues were subjected to western blot analysis of mouse CXCL2 protein expression. The experiments were repeated three times, and a representative blot is shown. d The fold changes in the mRNA levels of various chemokines in chemical-induced mucositis were analyzed by RT-qPCR. Samples were extracted from normal and inflamed mucosal tissues on day 5 after chemical stimulation. The data are presented as the means？±？SEMs (n？=？6) for each group. e Immunofluorescence staining for CXCL2 (red) in normal and inflamed mucosal tissues on day 5 after chemical stimulation. The experiments were repeated three times in individual mice. Nuclei were visualized through DAPI staining (blue). Scale bar？=？100？μm. f Total tissue lysates from normal and inflamed mucosal tissues were subjected to western blot analysis of mouse CXCL2. RIM radiation-induced mucositis, CIM chemical-induced mucositi