Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2

a Diagram of mouse Sox2ot locus. Green, Sox2ot probe; orange, Sox2 probe. Chr, chromosome. Exon 1 (E1) to 5 (E5) are labeled. b–e Low-power (top panels) and high-power (bottom panels) images of in situ hybridization in the cerebral cortex of wild-type mice at E13.5 and E15.5. The ventricular zone expression of Sox2 (arrowheads) and Sox2ot (arrows) is highlighted. f Ratio of Sox2ot/Sox2 expression in cDNA from the dorsal cortex at various developmental stages detected by droplet digital quantitative PCR (ddPCR). n？=？3 repeats. g Northern blot detecting Sox2 and Sox2ot in the RNA extracted from the E13.5 dorsal cortex (upper panels). Gapdh is shown as a loading control (lower panels