The long noncoding RNA GAS5 negatively regulates the adipogenic differentiation of MSCs by modulating the miR-18a/CTGF axis as a ceRNA

a Relative expression of GAS5 during the adipogenic differentiation of MSCs. b Relative expression levels of GAS5 and PPAR-γ during the adipogenic differentiation of MSCs obtained from 18 healthy donors. c, d ORO staining and quantification on days 0, 3, 7, 10, 14, and 18 of MSC adipogenic differentiation. e, f Relative levels of PPAR-γ, C/EBP-α and FABP4 mRNAs and proteins on days 0, 3, 7, 10, 14, and 18 of MSC adipogenic differentiation. The results are presented as the means？±？SD (*P？<？0.05, **P？<？0.01, as determined by Student’s t-test). All experiments were performed three independent time